rho activator ii Search Results


cn03  (Cytoskeleton Inc)
96
Cytoskeleton Inc cn03
NAD + acts on upstream of Rho activation to suppresses spheroid formation after injury. ( a ) Fluo4-AM calcium imaging of wild-type sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( b ) Quantification of axonal spheroid number per 100 µm of wild-type sympathetic axons at the indicated times after injury in the absence and presence of NAD + or <t>CN03.</t> Total number of n = 23 (Control), n = 13 (NAD + ), n = 21 (NAD + , CN03) axons from cultured neurons harvested from 3 independent litters were quantified. ( c ) Fluo4-AM calcium imaging of Sarm1 −/− sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( d ) Quantification of axonal spheroid number per 100 µm of Sarm1 −/− sympathetic axons at the indicated times after injury in the absence and presence of NAD + or CT04. Total number of n = 30 (Control), n = 16 (NAD + ), n = 25 (CT04) Sarm1 −/− axons from cultured neurons harvested from 3 independent litters were quantified. ( e ) Fluo4-AM calcium imaging of Wld s sympathetic axons 1 h after injury in the presence and absence of CN03. Scale bar = 10 µm. ( f ) Quantification of axonal spheroid number per 100 µm of Wld s sympathetic axons at the indicated times after injury in the absence and presence of CN03. Total number of n = 25 (Control), n = 24 (CN03) Wld s axons from cultured neurons harvested from 3 independent litters were quantified. ( g ) Representative images and ( h ) quantification of degeneration of wild-type and Wld s distal sympathetic axons immuno-stained for β3-tubulin with different treatments. Scale bar = 50 µm. For the “NAD + ” group, axons were incubated in SCG media containing 1 mM NAD + supplement overnight prior to injury. For the “CN03” and CT04″ groups, axons were incubated in SCG media containing 1 µg/mL Rho activator CN03 and Rho inhibitor CT04 for 2 h prior to injury, respectively. Data are reported as mean ± SEM, * p < 0.05; ** p < 0.001; *** p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test.
Cn03, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rho+activator+ii/pmc07508847-255-2-7?v=Cytoskeleton+Inc
Average 96 stars, based on 1 article reviews
cn03 - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
rho activator ii  (VANGL2 LTD)
90
VANGL2 LTD rho activator ii
NAD + acts on upstream of Rho activation to suppresses spheroid formation after injury. ( a ) Fluo4-AM calcium imaging of wild-type sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( b ) Quantification of axonal spheroid number per 100 µm of wild-type sympathetic axons at the indicated times after injury in the absence and presence of NAD + or <t>CN03.</t> Total number of n = 23 (Control), n = 13 (NAD + ), n = 21 (NAD + , CN03) axons from cultured neurons harvested from 3 independent litters were quantified. ( c ) Fluo4-AM calcium imaging of Sarm1 −/− sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( d ) Quantification of axonal spheroid number per 100 µm of Sarm1 −/− sympathetic axons at the indicated times after injury in the absence and presence of NAD + or CT04. Total number of n = 30 (Control), n = 16 (NAD + ), n = 25 (CT04) Sarm1 −/− axons from cultured neurons harvested from 3 independent litters were quantified. ( e ) Fluo4-AM calcium imaging of Wld s sympathetic axons 1 h after injury in the presence and absence of CN03. Scale bar = 10 µm. ( f ) Quantification of axonal spheroid number per 100 µm of Wld s sympathetic axons at the indicated times after injury in the absence and presence of CN03. Total number of n = 25 (Control), n = 24 (CN03) Wld s axons from cultured neurons harvested from 3 independent litters were quantified. ( g ) Representative images and ( h ) quantification of degeneration of wild-type and Wld s distal sympathetic axons immuno-stained for β3-tubulin with different treatments. Scale bar = 50 µm. For the “NAD + ” group, axons were incubated in SCG media containing 1 mM NAD + supplement overnight prior to injury. For the “CN03” and CT04″ groups, axons were incubated in SCG media containing 1 µg/mL Rho activator CN03 and Rho inhibitor CT04 for 2 h prior to injury, respectively. Data are reported as mean ± SEM, * p < 0.05; ** p < 0.001; *** p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test.
Rho Activator Ii, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rho+activator+ii/pmc07658195-91-5-17?v=VANGL2+LTD
Average 90 stars, based on 1 article reviews
rho activator ii - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


NAD + acts on upstream of Rho activation to suppresses spheroid formation after injury. ( a ) Fluo4-AM calcium imaging of wild-type sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( b ) Quantification of axonal spheroid number per 100 µm of wild-type sympathetic axons at the indicated times after injury in the absence and presence of NAD + or CN03. Total number of n = 23 (Control), n = 13 (NAD + ), n = 21 (NAD + , CN03) axons from cultured neurons harvested from 3 independent litters were quantified. ( c ) Fluo4-AM calcium imaging of Sarm1 −/− sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( d ) Quantification of axonal spheroid number per 100 µm of Sarm1 −/− sympathetic axons at the indicated times after injury in the absence and presence of NAD + or CT04. Total number of n = 30 (Control), n = 16 (NAD + ), n = 25 (CT04) Sarm1 −/− axons from cultured neurons harvested from 3 independent litters were quantified. ( e ) Fluo4-AM calcium imaging of Wld s sympathetic axons 1 h after injury in the presence and absence of CN03. Scale bar = 10 µm. ( f ) Quantification of axonal spheroid number per 100 µm of Wld s sympathetic axons at the indicated times after injury in the absence and presence of CN03. Total number of n = 25 (Control), n = 24 (CN03) Wld s axons from cultured neurons harvested from 3 independent litters were quantified. ( g ) Representative images and ( h ) quantification of degeneration of wild-type and Wld s distal sympathetic axons immuno-stained for β3-tubulin with different treatments. Scale bar = 50 µm. For the “NAD + ” group, axons were incubated in SCG media containing 1 mM NAD + supplement overnight prior to injury. For the “CN03” and CT04″ groups, axons were incubated in SCG media containing 1 µg/mL Rho activator CN03 and Rho inhibitor CT04 for 2 h prior to injury, respectively. Data are reported as mean ± SEM, * p < 0.05; ** p < 0.001; *** p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test.

Journal: Scientific Reports

Article Title: Regulation of degenerative spheroids after injury

doi: 10.1038/s41598-020-71906-x

Figure Lengend Snippet: NAD + acts on upstream of Rho activation to suppresses spheroid formation after injury. ( a ) Fluo4-AM calcium imaging of wild-type sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( b ) Quantification of axonal spheroid number per 100 µm of wild-type sympathetic axons at the indicated times after injury in the absence and presence of NAD + or CN03. Total number of n = 23 (Control), n = 13 (NAD + ), n = 21 (NAD + , CN03) axons from cultured neurons harvested from 3 independent litters were quantified. ( c ) Fluo4-AM calcium imaging of Sarm1 −/− sympathetic axons 1 h after injury with or without drug treatment. Scale bar = 10 µm. ( d ) Quantification of axonal spheroid number per 100 µm of Sarm1 −/− sympathetic axons at the indicated times after injury in the absence and presence of NAD + or CT04. Total number of n = 30 (Control), n = 16 (NAD + ), n = 25 (CT04) Sarm1 −/− axons from cultured neurons harvested from 3 independent litters were quantified. ( e ) Fluo4-AM calcium imaging of Wld s sympathetic axons 1 h after injury in the presence and absence of CN03. Scale bar = 10 µm. ( f ) Quantification of axonal spheroid number per 100 µm of Wld s sympathetic axons at the indicated times after injury in the absence and presence of CN03. Total number of n = 25 (Control), n = 24 (CN03) Wld s axons from cultured neurons harvested from 3 independent litters were quantified. ( g ) Representative images and ( h ) quantification of degeneration of wild-type and Wld s distal sympathetic axons immuno-stained for β3-tubulin with different treatments. Scale bar = 50 µm. For the “NAD + ” group, axons were incubated in SCG media containing 1 mM NAD + supplement overnight prior to injury. For the “CN03” and CT04″ groups, axons were incubated in SCG media containing 1 µg/mL Rho activator CN03 and Rho inhibitor CT04 for 2 h prior to injury, respectively. Data are reported as mean ± SEM, * p < 0.05; ** p < 0.001; *** p < 0.0001, two-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: CT04 and CN03 were the products of Cytoskeleton Inc. Cytochalasin D (C2618), cytosine arabinofuranoside (Ara-C), poly-D-lysine, and paraformaldehyde were purchased from Millipore Sigma.

Techniques: Activation Assay, Imaging, Control, Cell Culture, Staining, Incubation